Microfluidic chips, made of PDMS, are one-side open when fabricated. Another layer of glass, PDMS, or etc is needed. Liquid seal is provided by complete covalent bonding between layers or by external forces like threads, magnets or similar factors. This protocol describes the covalent bonding of PDMS on glass by air plasma technique.

This protocol is derived from "soft lithography" literature and can be modified by user freely.